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Laboratory for the Study of Atherosclerosis  

Responsible Scientist
Prof. Cesare R. Sirtori, M.D. ,Ph.D., Ordinary Professor
e-mail: cesare.sirtori@unimi.it

Research Team
Elisabetta Gianazza, Ph.D. Associate Professor
Maria Rosa Lovati,  Ph.D. Associate Professor
Cristina Manzoni, Ph.D. Technician
Anna Parolari, Doctorate Student

 

Research lines

The laboratory follows numerous research projects involving the basic  science of atherosclerosis. In particular, proteomic technologies  are being evaluated, in order to identify  cellular and molecular  disorders.  In addition, a better understanding of nutraceuticals regulating lipid metabolism, of common use, is being pursued.  Finally, a major area of research is  the pharmacology  of eicosanoids, with particular emphasis  on new non steroidal antiinflammatory drugs and on lipid metabolites from arachidonic acid.

 

Technologies

-  Proteomic technologies for studies on tissues and biological fluids
-  In silico characterization of proteins of pharmacological interest
-  Identification, biosynthesis and biological activity of peptides active on lipid metabolism, present in nutraceutical products
-  Methods for the  monitoring of the quality  of waters, sediments, muds and  soils before and after reclamation
-  Identification of biologically active eicosanoids in body fluids
-  Whole animal methods to evaluate cholesterol absorption/elimination and low density lipoprotein receptor regulation

 

Selected Publications

Bjellqvist B, Ek K, Righetti PG, Gianazza E, Görg A, Westermeier R, Postel W (1982) Isoelectric focusing in immobilized pH gradients: Principles, methodology and some applications. J Biochem Bio- phys Methods6: 317-9

Sirtori CR, Lovati MR, Manzoni C, Castiglioni S, Duranti M, Magni C, Morandi S, D’Agostina A, Arnoldi A (2004) Proteins  of white lupin seed, a  naturally isoflavone-poor legume,  reduce cholesterolemia  in rats and increase LDL receptor  activity in HepG2 cells. J Nutr134: 18-23

Gianazza E, Vergani L, Wait R, Brizio C, Brambilla D, Begum S, Giancaspero TA, Conserva F, Eberini I, Bufano D,  Angelini C, Pegoraro E, Tramontano A, Barile M (2006) Coordinated and reversible reduction of enzymes involved in terminal oxidative metabolism in skeletal muscle mitochondria from a riboflavin-responsive, multiple acyl-CoA dehydrogenase deficiency (RR-MAD) patient. Electrophoresis  27: 1182-8

Gomaraschi M, Baldassarre D, Amato M, Eligini S, Conca P,  Sirtori CR, Franceschini G, Calabresi L (2007) Normal vascular function despite low levels of high-density lipoprotein cholesterol in carriers of the apolipoprotein A-IMilano mutant. Circulation  116: 2165-72

Guerini Rocco A, Mollica L, Ricchiuto P, Baptista AM, Gianazza E, Eberini I (2008) Characterization of the protein unfolding processes induced by urea and temperature, Biophys J15: 2241-51 

Consonni A, Lovati MR, Manzoni M,  Pizzagalli A, Morazzoni P,  Duranti M (2010) Cloning, yeast expression, purification and biological activity of a truncated form of the soybean 7S globulin a’ subunit involved in HepG2 cell cholesterol homeostasis. J Nutr Biochem  21: 887-91

Consonni A, Lovati MR, Parolari A, Manzoni C, Morazzoni P, Magni C, Duranti M (2011) Heterologous expression and purification of the soybean 7S globulin a’ subunit extension region: In vitro evidence of its involvement in cell cholesterol homeostasis. Protein Express and Purification  80: 125-9

Lovati M.R, Manzoni C, Castiglioni S, Parolari A, Magni C, Duranti M (2012) Lupin seed g-conglutin lowers blood glucose in hyperglycaemic rats and increases glucose consumption of HepG2 cells. Brit J  Nutr  107: 67-73

Galli C, Maggi FM, Risè P, Sirtori CR (2012) Bioequivalence of two omega-3 fatty acid ethyl ester formulations: a case of clinical pharmacology of dietary: a case  of clinical pharmacology of dietary supplements. Br J Cin Pharmacol74: 60-5

Cuchel M, Meagher EA, du Toit Theran H, Blom DJ, Marais AD, Hegele RA. Averna MR,  Sirtori CR, Shah PK, Gaudet D,  Stefanutti C,  Vigna GB, Du Plessis AME, Propert KJ, Sasiela WJ, Bloedan LT, Reader DJ (2013) Efficacy and safety of a microsomal triglyceride transfer protein inhibitor in patients with homozygous  familial hypercholesterolemia: a single-arm, open-label, phase 3 study. Lancet  381: 40-6

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