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Laboratory of Clinical Pathology  

Lab. Magni

 

Head  Prof. Paolo Magni

Team
Massimiliano Ruscica, Assistant Professor
Liliana Steffani, Ph.D. student
Chiara Macchi, Research Fellow

 

Research fields

For over 20 year, the laboratory has been dealing with biomedical research in the area of "brain-gut" neuropeptides, with reference to the pathophysiology of reproduction, metabolism and cardiovascular diseases, as well as to endocrine oncology. Moreover, research has been conducted in the field of nutrition, nutraceutics and cardiometabolic risk. This laboratory collaborates with the E. Grossi Paoletti Centre for the study of metabolic diseases and atherosclerosis (Niguarda Cà Granda Hospital, Milan).

  • Molecular pathophysiology and clinical pathology of cardiovascular and endocrine-metabolic diseases (obesity, dyslipidemia, metabolic syndrome, cardiovascular risk). Use of in vitro and in vivo models, including humans.
  • Management of metabolic diseases by using nutraceutical approaches.
  • Molecular pathology in the progression of prostate cancer (peptidergic systems).

 

Techniques

  • Cell cultures
  • RNA, DNA and protein extractions
  • Real-Time (q)-PCR
  • Western blot and immunoprecipitation analysis
  • Immunofluorescence analysis
  • Boyden’s chamber migration assay (chemotaxis and chemokinesis)
  • Evaluation of plasma hormonal and inflammatory parameters by immunometric (ELISA) and immunoradiologic (RIA) assays
  • Evaluation of cell proliferation by bromodeoxyuridine DNA incorporation assay (BrdU)
  • Animals handling
  • Management of clinical trials

 

Selected publications

1. M. Ruscica, E. Dozio, S. Boghossian, G. Bovo, V. Martos Riaño, M. Motta, P. Magni (2006) Activation of Y1 receptors by neuropeptide Y regulates the growth of prostate cancer cells. Endocrinology147:1466-73.

2. P. Magni, E. Dozio, H. Watanobe, M. Ruscica, A. Cariboni, R. Zaninetti, M. Motta, R. Maggi (2007) Leukemia inhibitory factor induces the chemomigration of immortalized gonadotropin-releasing hormone neurons through the independent activation of the Janus kinase/signal transducer and activator of transcription 3, mitogen-activated protein kinase/extracellularly regulated kinase 1/2, and phosphatidylinositol 3-kinase/Akt signaling pathways. Mol Endocrinol21:1163-74,

3. Massimiliano Ruscica, Elena Dozio, Sara Gandini, Pierluigi Gnocchi, Guya Giuseppina Devalle, Marcella Motta, Elio Roti, Paolo Magni (2008) Total, free and bound leptin and thyroid function in elderly women with different body weights. Clin Endocrinol (Oxf.) 68:1002-08.

4. Magni P., Dozio E., Ruscica M., Celotti, F., Masini M.A., Prato P., Broccolo M., Mambro A., Morè M., Strollo F (2009) Feeding Behaviour in Mammals Including the Human. Ann N Y  Acad Sci1163:221-32.

5. M. Ruscica, M. Arvigo, F. Gatto, E. Dozio, D. Feltrin, M.D. Culler, F. Minuto, M. Motta, D. Ferone, P. Magni (2010) Regulation of prostate cancer cell proliferation by somatostatin receptor activation. Mol Cell Endocrinol315:254-62.

6. Magni P, Ruscica M, Dozio E, Passafaro L, Steffani L, Morelli P, Banfi G, Corsi MM (2010) Plasma adiponectin and leptin concentrations in professional rugby players. J Biol Regul Homeost Agents24:87-91.

7. Dozio E., Massimiliano Ruscica, Luca Passafaro, Giada Dogliotti, Liliana Steffani, Paola Marthyn, Alessandra Pagani, Germana Demartini, Daniele Esposti, Franco Fraschiniand Paolo Magni (2010) The natural antioxidant alpha-lipoic acid induces p27Kip1-dependent cell cycle arrest and apoptosis in MCF-7 human breast cancer cells.. Eur J Pharmacol  641:29-34

8. Ruscica M., Dozio E., Steffani L.,Passafaro L., Mazzer M., Castellano J.M., Motta M., Tena-Sempere M., Magni P (2011) Role of the energy sensor AMP-activated protein kinase in the regulation of immature GnRH neuron migration. J Endocrinol Invest34:e362-68.

9. Magni P., Ruscica M., Dozio E., Rizzi E., Beretta G., Maffei Facino R (2012)  Parthenolide inhibits the LPS-induced secretion of IL-6 and TNF-a and NF-κB nuclear translocation in BV-2 microglia. Phytoter Res26:1405-1409.

10. Massimiliano Ruscica, Marica Arvigo, Liliana Steffani, Diego Ferone, Paolo Magni (2013) Somatostatin, Somatostatin Analogs and Somatostatin Receptor 1 Dynamics in The Biology of Cancer Progression. Curr Mol Med13:555-571.

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