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Laboratory of Endocrine Pathophysiology  

Lab. Celotti

 

Principal investigator
Prof. Fabio Celotti, Full prof.  fabio.celotti@unimi.it

Team
Paola Negri Cesi, Associated professor  paola.negricesi@unimi.it
Alessandra Colciago, Research associated  alessandra.colciago@unimi.it
Lavinia Casati, Post doc student  lavinia.casati@unimi.it
Ornella Mornati, Technician  ornella.mornati@unimi.it

 

Research lines

Brief introduction

We are studying since many years the role of the hormonal milieu, both endogenous and modified by some endocrine disruptors, in different pathophysiological processes involved in CNS sexual differentiation and in cancer progression.

More recently, we are investigating the possible modulation of the inflammatory process in some crucial pathological phenomena, like tumor progression and tissue regeneration.

Ongoing research lines:

  1. Role of polychlorinated biphenyls (PCB) in the interplay between steroid receptors (particularly androgen receptors) and epigenome (especially chromatin remodeling enzymes) in a complex disease as autism spectrum disorder.
  2. Studies on bisphenol A (BPA): a- effects on immature GnRH neuron migration in vitro; b- effects of a prenatal exposure on the hypothalamic network controlling food intake; c- in vitro evaluation of the epigenetic effects in trophoblast cell lines (in collaboration with dr. Ricci, Life Sciences Dept., University of Siena).
  3. Possible application of high and low intensity electromagnetic field and of a platelet gel (alone or in combination) on tissue regeneration altered by a microgravity exposure (Granted by ASI-2011, “progetto RITMI”).
  4. Relationship between the inflammatory microenvironment and “in vitro” models of human melanoma (Granted by PRIN 2010-2011).
  5. Studies in vitro on the potency of estrogen receptor beta (ERbeta) selective ligands on prostate cancer cell proliferation
  6. Analysis of the cross-talk between epigenetic mechanisms and ERbeta; effect of nutraceutics (tocotrienols) in ERbeta-Epigenome interaction (in collaboration with dr. Limonta of our dept.)
  7. Evaluation of the effect of oxidative stress and GH secretagogue peptides on epigenetic pattern of primary culture of osteoblasts (in collaboration with dr Sibilia, BIOMETRA Dept., University of Milano)

 

Main techniques used

In the lab the following methodologies of cellular and molecular biology are currently used:

  1. Cell culture, transient transfection, proliferation assay (MTT, cell count), transcriptional activity (luciferase assay), immunofluorescence, migration assay (Boyden chamber), Elisa immunoassay.
  2. Bacterial transformation, cloning, selection of recombinant bacteria, plasmid amplification and cut, qualitative RT-PCR, Real Time PCR (TaQMan chemistry and SYBR), fluorescent Western blotting, chromatin and histones extraction, fluorescent WB of core histone, ChIP, methylation profiling by restriction enzymes, Methylation specific PCR.
  3. We also use the following software for primer design (Primer3 or primer Blast), for CpG analysis (Methyl primer Express), for statistical analysis (SPSS) and for the gene sequence analysis (Genomatix).

 

Selected publications (max 10 pubblicazioni)

  1. Casati L, Sendra R, Colciago A, Negri Cesi P, Berdasco M, Esteller M, Celotti F (2012) Polychlorinated biphenyls (PBC) affect the histone modification pattern in early development of rats: a possible role for androgen receptor-dependent modulation. Epigenome4:101-112.
  2. Colciago A, Casati L, Mornati O, Vergoni A.V, Santagostino A, Celotti F., Negri-Cesi P (2009) Chronic treatment with polychlorinated Biphenyls (PCB) during pregnancy and lactation in the rat Part 2: Effects on reproductive parameters, on sex behavior, on memory retention and on hypothalamic expression of aromatase and 5alpha-reductases in the offspring. Toxicol Applied Pharmacol239: 46-54.
  3. Cocchi D, Tulipano G, Colciago A, Sibilia V, Pagani F, Viganò D, Rubino T, Parolaro D, Bonfanti P, Colombo A, Celotti F (2009). Chronic treatment with polychlorinated biphenyls (PCB) during pregnancy and lactation in the rat Part 1: Effects on somatic growth, Growth hormone-axis activity and bone mass in the offspring. Toxicol Applied Pharmacol237:127-136.
  4. Colciago A, Celotti F, Casati L, Giancola R, Castano SM, Antonini G, Sacchi MC, Negri-Cesi P (2009) In Vitro Effects of PDGF Isoforms (AA, BB, AB and CC) on migration and proliferation of SaOS-2 osteoblasts and on migration of human osteoblasts. Int J Biomedical Sci5:380-389.
  5. Negri-Cesi P, Colciago A, Pravettoni A, Casati L, Conti L, Celotti F (2008). Sexual differentiation of the rodent hypothalamus: hormonal and environmental influences. J Steroid Biochem Molec Biol109: 294-299.
  6. Pravettoni A, Mornati O, Martini PGV, Marino M, Colciago A, Celotti F, Motta M, Negri-Cesi P (2007). Estrogen receptor beta (erbeta) and inhibition of prostate cancer cell proliferation: studies on the possible mechanism of action in DU145 cells. Molecular Cell Endocrinol263:46-54.
  7. Colciago A, Negri-Cesi P, Pravettoni A, Mornati O, Casati L, Celotti F (2006). Prenatal Aroclor 1254 exposure and brain sexual Differentiation: Effect on the expression of testosterone metabolizing enzymes and androgen receptors in the hypothalamus of male and female rats. Reproductive Toxicol22:738-745.
  8. Colciago A, Celotti F, Pravettoni A, Mornati O, Martini L, Negri-Cesi P (2005). Dimorphic expression of testosterone metabolizing Enzymes in the hypothalamic area of developing rats. Brain Res. Develop Brain Res155:107-116.
  9. Pravettoni A, Colciago A, Negri-Cesi P, Villa S, Celotti F (2005). Ontogenetic development, sexual differentiation and effects of aroclor 1254 exposure on the expression of the arylhydrocarbon receptor and of the arylhydrocarbon receptor nuclear translocator in the rat hypothalamus. Reproductive Toxicol20:521-531.
  10. Poletti A, Negri-Cesi P, Rabbuffetti M, Colciago A, Celotti F, Martini L (1998). Transient expression of the 5alpha-reduciase type 2 isozyme in the rat brain in the late fetal and early post-natal life. Endocrinology139:2171-2178.

 

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