Pathophysiology of pain, neuroimmunology and drug of abuse Unit
1)The laboratory is involved in the study of the mechanisms at the basis of onset, maintenance and resolution of chronic inflammatory and neuropathic pain. We and others recently demonstrated that a pathological interaction between neurons, glia, microglia and immune cells in the peripheral and central nervous system underlies the chronicization of pain. Our research aims at identifying the molecular and cellular mechanisms and the main determinants at the basis of neuroinflammatory processes in the nervous system sites involved in pain transmission, focussing on the pro-anti-inflammatory cytokine balance. A deep understanding of these interactions may indicate novel therapeutic targets for treating neuropathic pain, that is refractory to most known analgesics.
In particular the lab is studying the role of a novel family of chemokines, the prokineticins, that are pivotal players at the cross roads between neuroinflammation and pain.
As innovative therapeutic approach to pain we are using stem cells, secretome and microvescicles to modulate the activity of resident cells , also along the pathways involved in pain transmission.
2)Marijuana and opioids are wide spread substances of abuse, that induce a large range of diverse effects including their ability to modify the immune system responses. Our laboratory evaluates the effects that opioids and cannabis derivatives have on immune responses, both in vitro and in vivo in the human and in animal models, in order to identify their effect on health.
Behavioural laboratory for the evaluation of nociception, allodynia and hyperalgesia
Animal models of inflammatory and neuropathic pain
Cellular immunology (lymphoproliferation, chemotaxis, cytokine production, antibody production)
Primary cultures of sensory neurons
The laboratory has many collaborations with national and International groups working in the field of pain.
AMODEO G, BUGADA D, FRANCHI S, MOSCHETTI G, GRIMALDI S, PANERAI A, ALLEGRI M, SACERDOTE P.
Immune function after major surgical interventions: the effect of postoperative pain treatment JOURNAL OF PAIN RESEARCH, 11: 1297–1305, 2018
BRINI A, AMODEO G, FERREIRA LM, MILANI A, NIADA S, MOSCHETTI G, FRANCHI S, BORSANI E, RODELLA LF, PANERAI AE, SACERDOTE P.
Therapeutic effect of human adipose-derived stem cells and their secretome in experimental diabetic pain SCIENTIFIC REPORTS, Sci Rep. 2017 Aug 29;7(1):9904
CASTELLI M, AMODEO G, NEGRI L, LATTANZI R, MAFTEI D, GOTTI C, PISTILLO F, ONNIS V, CONGU C, PANERAI AE, SACERDOTE P, FRANCHI S.
Antagonism of the Prokineticin System Prevents and Reverses Allodynia and Inflammation in a Mouse Model of Diabetes. PLOS ONE. 2016 Jan 5;11(1):e0146259
MORETTI S, CASTELLI M, FRANCHI S, RAGGI MA, MERCOLINI L,PROTTI M, SOMAINI L,PANERAI AE, SACERDOTE P.
Delta 9-Tetrahydrocannabinol-induced anti-inflammatory responses in adolescent mice switch to proinflammatory in adulthood J. LEUKOCYTE BIOL 96:523-34 2014
FRANCHI S, VALSECCHI AE, BORSANI E, PROCACCI P, FERRARI D, ZAFFA C, SARTORI P, RODELLA LF, VESCOVI A, MAIONE S, ROSSI F, COLLEONI M, SACERDOTE P, PANERAI AE. Intravenous Neural Stem Cells abolish nociceptive hypersensitivity and trigger nerve regeneration in experimental neuropathy. Pain, 153:850-861 2012